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Influence of long-term fertilization on soil microbial biomass, dehydrogenase activity, and bacterial and fungal community structure in a brown soil of northeast China

http://download.springer.com/static/pdf/628/art%253A10.1007%252Fs13213-014-0889-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs13213-014-0889-9&token2=exp=1447219458~acl=%2Fstatic%2Fpdf%2F628%2Fart%25253A10.1007%25252Fs13213-014-0889-9.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs13213-014-0889-9*~hmac=21de115a0f5ecfeddb22ede3a9bbdf789770d2ea17f0145140f3b22340007494
By: Peiyu Luo; Xiaori Han; Yan Wang; Mei Han; Hui Shi; Ning Liu and Hongzhi Bai
Annals of Microbiology --- Year 2015; Volume 65; Issue 1; Page 533-542

http://download.springer.com/static/pdf/628/art%253A10.1007%252Fs13213-014-0889-9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs13213-014-0889-9&token2=exp=1447219458~acl=%2Fstatic%2Fpdf%2F628%2Fart%25253A10.1007%25252Fs13213-014-0889-9.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs13213-014-0889-9*~hmac=21de115a0f5ecfeddb22ede3a9bbdf789770d2ea17f0145140f3b22340007494Keywords: Long-term fertilization; Bacterial and fungal community structure; Soil microbial biomass carbon; Dehydrogenase activity; PCR-DGGE

Abstract
In this study, the effect of mineral fertilizer and organic manure were evaluated on soil microbial biomass, dehydrogenase activity, bacterial and fungal community structure in a long-term (33 years) field experiment. Except for the mineral nitrogen fertilizer (N) treatment, long-term fertilization greatly increased soil microbial biomass carbon (SMBC) and dehydrogenase activity. Organic manure had a significantly greater impact on SMBC and dehydrogenase activity, compared with mineral fertilizers. Bacterial and fungal community structure was analyzed by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Long-term fertilization increased bacterial and fungal ribotype diversity. Total soil nitrogen (TN) and phosphorus (TP), soil organic carbon (SOC) and available phosphorus (AP) had a similar level of influence on bacterial ribotypes while TN, SOC and AP had a larger influence than alkali-hydrolyzable nitrogen (AHN) on fungal ribotypes. Our results suggested that long-term P-deficiency fertilization can significantly decrease soil microbial biomass, dehydrogenase activity and bacterial diversity. N-fertilizer and SOC have an important influence on bacterial and fungal communities.